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Department of Enteric Diseases: Research Projects

  Clinical & Preclinical active
Title: Establishment of a Shigella sonnei Challenge Model for evaluation of future vaccine candidates
Description: A clinical study to evaluate the optimal challenge dose of S. sonnei in Thai healthy adult volunteers. Studies are planned to follow this study to evaluate the safety, immunogenicity, and efficacy of candidate S. sonnei vaccines.
Collaborator: Trial Center, Faculty of Tropical Medicine, Mahidol University
Link: http://www.tm.mahidol.ac.th/en/vtc/vtcmainframe.html
Funding: NIAID
  Clinical & Preclinical active
Title:

Immunogenicity and efficacy of a Shigella dysenteriae-1 vaccine candidate (WRSd1) in non-human primates
Description: WRSd1 is an established live attenuated S. dysenteriae-1 vaccine candidate.  This study examines the safety, and immunogenicity, and efficacy of the WRSd1 vaccine in rhesus monkeys.  This project will establish the baseline for evaluation of other recently developed S. dysenteriae vaccines.
Collaborator: Department of Veterinary Medicine, AFRIMS and Walter Reed Army Institute of Research (WRAIR)
Link:

http://www.afrims.org/vetmed.html
http://wrair-www.army.mil
Funding: NIAID
  Epidemiology active
Title:

Etiology of acute diarrhea in children and adults in Maldives
Description: Hospital based surveillance of diarrhea etiology and antimicrobial resistant enteric pathogens.
Collaborator: Maldives Ministry of Health, Indira Gandhi Memorial Hospital, Male, Republic of Maldives
Link: http://www.igmh.gov.mv
Funding: DoD-GEIS
  Epidemiology active
Title:

Etiology of diarrhea in children in Bharatpur, Nepal
Description: Hospital based surveillance of diarrhea etiology and antimicrobial resistant enteric pathogens in young Nepalese children.
Collaborator: Bharatpur Hospital, Bharatpur, Nepal; Walter Reed AFRIMS Research Unit, Nepal (WARUN)
Link: http://www.afrims.org/warun.html
Funding: DoD-GEIS
  Epidemiology active
Title:

Etiology of diarrhea in children attending Kanti Children Hospital in Kathmandu, Nepal
Description: Hospital based surveillance of diarrhea etiology and antimicrobial resistant enteric pathogens in young Nepalese children.
Collaborator: Kanti Children Hospital, Kathmandu, Nepal; Walter Reed AFRIMS Research Unit, Nepal (WARUN)
Link: http://www.afrims.org/warun.html
Funding: DoD-GEIS
  Epidemiology active
Title:

Etiology of diarrhea in adults attending Sukraraj Tropical and Infectious Diseases Hospital Kathmandu, Nepal
Description: Hospital based surveillance of diarrhea etiology and antimicrobial resistant enteric pathogens in Nepalese adults.
Collaborator: Sukraraj Tropical and Infectious Diseases Hospital, Kathmandu, Nepal; Walter Reed AFRIMS Research Unit, Nepal (WARUN)
Link: http://www.afrims.org/warun.html
Funding: DoD-GEIS
  Epidemiology active
Title:

Etiology of diarrhea in children attending Phramongkutklao Hospital in Bangkok
Description: Hospital based surveillance of diarrhea etiology and antimicrobial resistant enteric pathogens in urban Thailand
Collaborator: Phramongkutklao Hospital
Link: http://golden.pmk.ac.th/
Funding: DoD-GEIS
  Epidemiology active
Title:

Etiology of diarrhea in children attending Queen Sirikit National Institute of Child Health in Bangkok
Description: Hospital based surveillance of diarrhea etiology and antimicrobial resistant enteric pathogens in urban Thailand
Collaborator: Queen Sirikit National Institute of Child Health in Bangkok
Link: http://www.childrenhospital.go.th/main/index.php
Funding: DoD-GEIS
  Epidemiology active
Title:

Regional Surveillance of Diarrhea etiology in Thai Children
Description: Hospital based surveillance of diarrhea etiology and antimicrobial resistance in bacterial enteric pathogens in rural Thailand
Collaborator: Department of Medical Sciences, Ministry of Public Health, Thailand; Regional Medical Sciences Centers, provincial and district hospitals in 5 different regions of Thailand
Link: http://www.dmsc.moph.go.th/ http://webdb.dmsc.moph.go.th/ifc_nih/ez.mm_main.asp
Funding: DoD-GEIS
  Diagnostics active
Title:

Evaluation of the Cape Town protocol for isolation of Campylobacter species from diarrhea stool specimens.
Description:

Diarrheal stool specimens are processed for the isolation of Campylobacter by multiple techniques.  The Cape Town protocol was developed to better isolate unusual Campylobacter spp. putatively associated with human diarrheal disease.
Collaborator: Walter Reed Army Institute of Research
Link: http://wrair-www.army.mil
Funding: MIDRP
  Diagnostics active
Title:

Evaluation of Real-time PCR assays development for detection of enteric pathogens
Description: Real-time PCR assays are applied to clinical stool samples for detection of enteric pathogens including Shigella spp., Campylobacter spp., Salmonella, diarrheogenic E. coli, Vibrio cholera, Cyclospora and Cryptosporidium parvum and Noroviruses.
Collaborator: Walter Reed Army Institute of Research
Link: http://wrair-www.army.mil
Funding: MIDRP
  Applied Science active
Title:

Characterization of Campylobacter jejuni isolates from Thailand
Description: Sequencing of genes for virulence factors, candidate antigens, lipooligosaccharide (LOS) synthesis from human C. jejuni isolates from Thailand
Collaborator: Naval Medical Research Center, Silver Spring, MD, USA.
Link: http://www.nmrc.navy.mil/nmrc_id_ed.htm
Funding: MIDRP
  Applied Science active
Title:

Molecular typing of Campylobacter jejuni isolates from Thailand
Description: Comparison of two molecular typing techniques (multilocus sequence typing (MLST) and pulsed-field gel electrophoresis( PFGE)) and two serotyping assays for C. jejuni isolated from Thailand
Collaborator: Naval Medical Research Center, Silver Spring, MD, USA and Enteric Disease Program, National Microbiology Laboratory, Winnipeg, Manitoba, Canada
Link: http://www.nmrc.navy.mil/nmrc_id_ed.htm
http://www.nml-lnm.gc.ca/english/entericsED_e.htm
Funding: MIDRP
  Applied Science active
Title:

Characterization of multi resistant Salmonella typhi isolated from Nepal
Description: Determination of antibiotic susceptibility patterns and sequencing of the GyrA, Gyr B, ParC and ParE genes of multiple drug resistant S. typhi isolates
Collaborator: National Public Health Laboratory, Teku, Kathmandu, Nepal and Bharatpur Hospital, Bharatpur, Nepal
Link: http://www.phclab.com
Funding: DoD-GEIS
  Applied Science active
Title:

Phylogenetic analysis of Noroviruses from Thailand
Description: of the genome of Norovirus isolates from Thailand are sequenced to better understand the phylogenetic relationships and the genetic diversity of Norovirus in Thailand and the region.
Collaborator: Walter Reed Army Institute of Research
Link: http://wrair-www.army.mil
Funding: MIDRP

 

Recently Completed Projects
  Epidemiology active
Title: Establishment of the intragastric challenge model for wild type Shigella dysenteriae 1 strain 1617 in rhesus monkeys
Description: To determine the challenge dose of wild type S. dysenteriae-1 1617 to reproducibly induce dysentery in the rhesus monkey model.  This project will provide the for the preclinical evaluation of S. dysenteriae vaccines.
Collaborator: Department of Veterinary Medicine, AFRIMS and Walter Reed Army Institute of Research (WRAIR)
Link: http://www.afrims.org/vetmed.html
http://wrair-www.army.mil
Funding: NIAID
  Epidemiology active
Title:

Etiology of diarrhea in children in Phnom Penh, Cambodia
Description: Hospital based surveillance of diarrhea etiology and antimicrobial resistant enteric pathogens
Collaborator: National Pediatric hospital, Phnom Penh, Cambodia
Link:

http://www.nphcambodia.org/
Funding: DoD-GEIS
  Epidemiology active
Title:

A multi-center study of prevalence and drug resistant pattern of Campylobacter and other enteric pathogens in Thailand
Description: Hospital based surveillance of diarrhea etiology and antimicrobial resistant enteric pathogens in rural Thailand
Collaborator: Department of Medical Sciences, Ministry of Public Health, Thailand; Regional Medical Sciences Centers, provincial and district hospitals in 5 different regions of Thailand
Link: http://www.dmsc.moph.go.th/ http://webdb.dmsc.moph.go.th/ifc_nih/ez.mm_main.asp
Funding: DoD-GEIS
  Diagnostics active
Title: Real-time PCR assay development for detection of enteric pathogens
Description: Real-time PCR assays were developed for detection of enteric pathogens including Shigella spp., Campylobacter spp., Salmonella, Virulence E. coli, Vibrio cholera, Cyclospora and Cryptosporidium parvum, and Noroviruses, Rotavirus.
Collaborator: Walter Reed Army Institute of Research
Link: http://wrair-www.army.mil
Funding: MIDRP
  Diagnostics active
Title:

Evaluation of a commercially available enzyme-linked immunosorbent assay (ELISA) for the detection of Giardia lamblia and Cryptosporidium spp from stool specimens.
Description: Stool specimens collected from October 2001 through October 2002  for the detection of Giardia lamblia and Cryptosporidium spp. antigens by enzyme-linked immunosorbent assay, stool microscopic examination and concentration techniques in order to compare the sensitivity of the each methods.
Collaborator: The Kwai River Christian Hospital, Sangklaburi district, Kanchanaburi, Thailand
Link: http://www.geocities.com/burmeseinn/Mission_Hospital.html http://www.twogypsies.com/html/what_krch_does.html
Funding: DoD-GEIS
  Diagnostics active
Title:

Comparison of Isolation Methods for Salmonella spp. from Adult Diarrheal Stool Samples
Description: Different primary, secondary plating media and enrichment broths were studied in order to improve isolation method for Salmonella species from stool specimens.
Collaborator: Walter Reed Army Institute of Research
Link: http://wrair-www.army.mil
Funding: MIDRP
  Applied Science active
Title:

Characterization of Vibrio parahaemolyticus isolates from Thailand
Description: V. parahaemolyticus isolates from Thailand were characterized by pulsed-field gel electrophoresis (PFGE),  polymerase chain reaction (PCR), and serotyping.
Collaborator: International Centre for Diarrhoeal Disease Research (ICDDR,B) Dhaka, Bangladesh and Bumrungrad Hospital, Bangkok, Thailand.
Link:

http://www.icddrb.org
http://www.bumrungrad.com
Funding: DoD-GEIS
  Applied Science active
Title:

Characterization of macrolide resistant and fluoloquinolone resistant among C. jejuni and C. coli isolates from Thailand
Description: Statistical and trends analysis of historical data on fluoroquinolone and macrolide resistant C. jejuni and C. coli isolated from Thailand.  Sequence analysis of 23S rDNA of macrolide resistant C. jejuni and C. coli isolated from Thailand.
Collaborator: Danish Institute for Food and Veterinary Research, Denmark and Department of Veterinary Pathobiology, Faculty of Life Sciences, Copenhagen University, Denmark
Link: http://www.food.dtu.dk
http://www.kvl.dk/English.aspx
Funding: MIDRP
  Applied Science active
Title:

Amplified fragment length polymorphism (AFLP) analysis of Shigella and Enterotoxigenic E. coli
Description: Application of AFLP to characterize untypable Shigella flexneri and colonization factor (CF) negative enterotoxigenic E. coli (ETEC)
Collaborator: WRAIR and Goteborg University
Link: http://wrair-www.army.mil/
http://www.biomedicine.gu.se/avdelningar/avdelning_2/
Funding: MIDRP

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